Technical Data
R1310-02
Red Fluorescent Protein (RFP)
Description:
Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression systems.
RFP-tags are composed of 15 amino acid residue peptides derived from aa22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.1-1.0ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceRabbit
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Peptide corresponding to aa22-36, VNGHEFEIEGEGEGR from the Discosoma sea anemone, conjugated to KLH
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitue with 80ul sterile ddH2O.
Specificity:
Recognizes fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. Does not react with GFP or GFP-tagged proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.