Technical Data
Resistin-like Molecule, gamma, Recombinant, Mouse (RELMg)
Molecular Biology Storage: -20CShipping: Blue Ice
RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats. Microarray and real-time RT-PCR experiments revealed a repression of RELMgamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM-gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.

The Recombinant Mouse RELM-gamma is a His -Tagged Fusion Protein having a molecular weight of 11kD containing 86 amino acid residues of the Mouse RELM-gamma and 16 additional amino acid residues HisTag (bold). MRGSHHHHHH GMASHMTLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA

The amino acid sequence of the recombinant Mouse RELM-gamma is 100% homologous to the amino acid sequence of the Mouse RELM-g.

Western blotting, ELISA

Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.

Store lyophilized protein at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized protein remains stable until the expiry date when stored at -20C.

Molecular Weight:
Source: E. coli
Purity: 95% (SDS-PAGE); Two-step procedure using size exclusion chromatography before and after refolding.
Concentration: ~0.5mg/ml
Form: Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Wang ML, Shin ME, Knight PA, Artis D, Silberg DG, Suh E, Wu GD. Regulation of RELM/FIZZ Isoform Expression by Cdx2 in Response to Innate and Adaptive Immune Stimulation in the Intestine. Am J Physiol Gastrointest Liver Physiol. 2004 Dec 2

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.