Technical Data
Respiratory Syncytial Virus (HRP)
Respiratory syncytial virus (RSV) is a negative-sense, single-stranded RNA virus and member of the Paramyxoviridae family. RSV causes respiratory tract infections in patients of all ages, but particularly affects infants and the immunosuppressed. RSV encodes three envelope glycoproteins, a small hydrophobic (SH) protein of unknown function, a glycoprotein (G) known as the attachment protein, and a fusion (F) protein. The F protein directs fusion of viral and cellular membranes, resulting in viral penetration, and can lead to the formation of syncytia. The F protein is thought to be the principal antigen responsible for inducing an immune response.

Suitable for use in ELISA, Immunofluorescence, and Immunohistochemistry (frozen and paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:20-1:200
ELISA: 1:200-1:1,000
Immunofluorescence: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
1ml-20CBlue IceGoat
Human RSV isolate.
Supplied as a liquid in PBS, 1% BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP).
Recognizes Respiratory Syncytial Virus. Does not react with Parainfluenza 1-3, Influenza A and B, Adenovirus or uninfected HEp-2 or WI-38 cells. It is neutralizing and reacts well with bovine isolates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.