Technical Data
R2079
RNA 5.8s, Ribosomal (Ribosomal RNA 5.8s, rRNA 5.8s)
Description:
The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.

This antibody is useful for recognizing RNA epitopes on both the large and small subunit.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Cellular Localization: cytoplasmic, nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG3,k9C93Affinity Purified
SizeStorageShippingSourceHost
100ul4C Do Not FreezeBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
The whole 5.8S ribosomal RNA (see reference 11).
Purity:
Purified by Protein G affinity chromatography.
Form
As reported
Specificity:
This antibody is specific for ribosomal RNA. There is no crossreactivity with other RNAs. Species Crossreactivity: This antibody reacts with all eukaryotes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.