Technical Data
R6038
Radical fringe homolog (Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide-3-beta-N-acetylglucosaminyltransferase, RFNG)
Description:
The Drosophila melanogaster 'fringe' gene is a secreted signaling protein that is involved in the formation of boundaries between groups of cells during development. It acts by modulating the activation of te Notch signal transduction pathway. A human radical fringe homolog was identified in 1997. There is evidence to show that radical fringe homolog may be involve in limb formation and in neurogenesis in humans. It is a glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. It is a single-pass type II membrane protein which localises to the golgi apparatus membrane. It belongs to the glycosyltransferase 31 family.

Applications:
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 10 ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20C. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9G432Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Partial recombinant human radical fringe homolog (aa222-330) with a GST tag.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human radical fringe homolog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.