Technical Data
R9700-06C
Rubella, E2 (German Measles)
Description:
Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It is an enveloped (toga=cloak), non-segmented, positive sense, RNA virus and replicates in the cytoplasm. It consists of 3 structural proteins; E1,E2 membrane bound glycoproteins, and C capsid protein. The the two envelope glycoproteins E2 and E1 are found as a heterodimeric spike complex embedded in the lipid envelope. E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein.

Rubella or German measles is a mild disease in children and adults, but can cause devastating problems if it infects the fetus, especially if infection is in the first few weeks of pregnancy.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k9L637Ascites
SizeStorageShippingSourceHost
1ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Rubella (E2).
Purity:
Ascites
Form
Supplied as a liquid in 0.02M Boric Acid, 5mM Sodium Borate, 0.2M Sodium Chloride, pH 8.0+0.2, 0.1% Sodium azide.
Specificity:
Recognizes E2 and reacts with reduced or non-reduced antigens on Western blots. Does not have HI activity. Low level neutralization of infectivity.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhu, Z., et al., (2007), "Comparison of Four Methods Using Throat Swabs to Confirm Rubella Virus Infection", Journal of Clinical Microbiology, 45(9): 2847-2852.