Description:
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.
Applications:
Suitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilutions:
Immunofluorescence: 1:10-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
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| Size | | Storage | Shipping | Source | Host |
|---|
| 250ul | | -20°C | Blue Ice | | Rabbit |
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| Concentration: |
| ~4mg/ml |
| Immunogen: |
| Mixture of S. enteriditis, S. typhimurium and S. heidelburg. Cellular Localization: Whole cell |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Specificity: |
| Recognizes Salmonella. Polyvalent for Salmonella O and H antigens. Does not react with related Enterobacteriaceae. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
General References:
1. Giannella RA (1996). Salmonella. In: Baron's Medical Microbiology (Barron S et al., eds.) (4th ed.). Univ of Texas Medical Branch. (via NCBI Bookshelf) ISBN 0-9631172-1-1.
2. Ryan KJ; Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 0-8385-8529-9.
3. Parkhill J et al. (2001). "Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18". Nature 413 (6858): 848–52. doi:10.1038/35101607. PMID 11677608 doi:10.1038/35101607.
4. McClelland M et al. (2001). "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2". Nature 413 (6858): 852–6. doi:10.1038/35101614. PMID 11677609 doi:10.1038/35101614.
5. Adkins JN et al. (2006). "Analysis of the Salmonella typhimurium Proteome through Environmental Response toward Infectious Conditions". Molecular and Cellular Proteomics 5: 1450–1461. doi:10.1074/mcp.M600139-MCP200. PMID 16684765.
6. Huang H, Li J, Yang XL, et al. (January 2009). "Sequence Analysis of the Plasmid pGY1 Harbored in Salmonella enterica Serovar Paratyphi A". Biochem. Genet. 47: 191. doi:10.1007/s10528-008-9216-0. PMID 19169860.
7. Swanson SJ, Snider C, Braden CR, et al. (2007). "Multidrug-resistant Salmonella enterica serotype Typhimurium associated with pet rodents". New England Journal of Medicine 356 (1): 21–28. doi:10.1056/NEJMoa060465. PMID 17202452.
8. Hensel M (2009). "Secreted Proteins and Virulence in Salmonella enterica". Bacterial Secreted Proteins: Secretory Mechanisms and Role in Pathogenesis. Caister Academic Press. ISBN 978-1-904455-42-4.
http://en.wikipedia.org/wiki/Salmonella_typhimurium
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