Technical Data
Salmonella enteritidis
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram-negative bacilli that are facultative and flagellated (motile).

Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors.

X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
2ml-20°CBlue IceMouse
Not determined
Flagellar preparation of S. enteritidis.
Supplied as a liquid, 0.09% sodium azide.
Recognizes S. enteritidis-100%; S. enteritidis PT4 strains-33-89%; S. agona-78%; S. typhimurium-5%; S. infantis-4%; S. hadar-1%; S. virchow-3%; Enterobacter cloacae-3%; E. coli-1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lee, H.A., et al., (1989), “Rapid enzyme-linked immunosorbent assays for the detection of Salmonella enteritidis in eggs”, Food and Agriculture Immunology, 1: 89-99.