Technical Data
SAPK4, p38 delta, active, Recombinant, Human (Stress-activated Protein Kinase 4, Mitogen-activated Protein Kinase 13, MAP Kinase 13, MAPK 13, MAPK13, PRKM13, Mitogen-activated Protein Kinase p38 delta, MAP Kinase p38 delta, p38delta)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant GST fusion protein fused to full length human SAPK4, expressed in E. coli.

Human stress-activated protein kinase-4 (SAPK4) is a novel MAP Kinase family member whose amino acid sequence is 60% identical to that of the other three SAP Kinases which contain a TGY motif in their activation domain. Recent results suggest that cellular functions attributed to SAPK1 and/or SAPK2 may be mediated by either SAPK3 or SAPK4.

Specific Activity: ~100U/mg where 1unit pf p38 delta/SAPK4 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein/minute at 30C with a final ATP of 100uM.

Kinase Assay: Enzyme was tested in a functional kinase assay by using 10-100ng of active SAPK4 to phosphorylate MBP in vitro.

Storage and Stability: Stable for 6 months at -20C from date of shipment.

Molecular Weight:
Source: Human recombinant expressed in E. coli
(full length).
Purity: Purified to 50% by affinity chromatography on glutathione Sepharose beads after activation with SKK3/MEK6.
Concentration: ~0.1mg/ml
Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol, and 50% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Goedert, M., et al., EMBO Journal: 16: 35633571, 1997.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.