		Technical Data
S0098-08Q	SARS M Protein (NT) (Severe Acute Respiratory Syndrome, Matrix Glycoprotein)

Description:
A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The M protein (Membrane protein, Matrix protein) is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein and the nucleocapsid protein.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to amino acids at the N-terminus of the SARS M protein.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes the N-terminal of the M protein of SARS-associated coronavirus.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Marra, MA., et al., (2003), "The Genome sequence of the SARS-associated corona virus", Science, 300, 1399-1404. 2. Rota, P.A., et al., (2003), "Characterization of a novel coronavirus associated with severe acute respiratory syndrome", Science, 300, 1394-1399. 3. Navas-Martin, S.R., et al., (2004), Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development", J. Neurovirol., 10, 75-85. 4. Arbely, E., et al., (2004), "A highly unusual palindromic transmembrane helical hairpin formed by SARS coronavirus E Protein", J. Mol. Biol., 3414, 769-779. 5. Maeda, J. et al., (1999), "Release of coronavirus E protein in membrane vesicles from virus-infected cells and E protein-expressing cells", Virology, 263, 265-272.