Technical Data
Media
Schaedler Broth (Powder)
S0450-05
Microbiological Grade
Storage RT/4C    Shipping RT

Schaedler Broth was first described by Schaedler et al. as an enriched medium for the isolation of anaerobes from mixed bacteria flora from the gastrointestinal track of mice. Mata et al., substituting Trypto Soy Broth for casein digest peptone in the Schaedler formulation, found that this medium gave excellent recovery of anaerobic and microaerophilic organisms from human fecal and gastrointestinal specimens. Schaedler Broth gave the highest rate of growth and response of anaerobic bacteria of nine broth media studied by Stalons et al.. General growth requirements are supplied by Trypto Soy Broth, dipeptone, yeast extract and dextrose. Hemin and cystine are added for specific requirements of some anaerobes. Blood and other enrichments may be added to enhance growth characteristics.

Appearence:
Light tan, free-flowing, homogeneous.

pH:
7.6 0.2

Directions per Liter:
Dissolve 28.4 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.



Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.