Technical Data
Scl-70 (Scleroderma, DNA Topoisomerase I)
Topoisomerase I changes the topology of DNA via cleavage of single strand only. Suitable for use in DNA conformational analysis and topology as well as DNA repair, drug resistance, cell proliferation and leukemia studies. The double-helical configuration that DNA strands naturally reside in makes them difficult to separate, and yet they must be separated if enzymes are to transcribe the sequences that encode proteins, or if chromosomes are to be replicated. In so-called circular DNA, in which double helical segment is bent around and joined in a circle, the two strands are topologically linked, or knotted. They cannot be separated by any process that does not involve the breaking of strands. Topoisomerases catalyze and guide the unknotting of DNA.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k4j81Affinity Purified
100ug-20CBlue IceHumanMouse
Full length Scl-70. Epitiope: YFVPPKEDFK.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.0, 0.02% sodium azide.
Recognizes human Scl-70.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.