|Molecular Biology||Storage: -20°CShipping: Blue Ice|
T T A A 5'-G G G C C^C-3'
3'-C^C C G G G-5' T T A A
Source: Streptococcus durans RFL3
Catalog No: R1625-75: Restriction Enzyme Buffer for SduI, 10X
10mM Tris-HCl (pH 7.2), 3mM MgCl2, 150mM NaCl and 0.1mg/ml BSA. Incubate at 37°C
Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with SduI (see Star Activity).
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with SduI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.4uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Star Activity: A large excess of enzyme (10u/ug DNA x 16 hours), low salt concentration or high pH may result in star activity.
Stability during Prolonged Incubation: A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min.
Compatible Ends: Alw21I, ApaI, Eco24I, Mph1103I, PstI, SacI, SdaI
Number of Recognition Sites in DNA:
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.