Technical Data
S0577
SduI
500U
Molecular Biology Storage: -20CShipping: Blue Ice
T T A A 5'-G G G C C^C-3'
3'-C^C C G G G-5' T T A A

Concentration: 10u/ul
Source: Streptococcus durans RFL3

Supplied with:
Catalog No: R1625-75: Restriction Enzyme Buffer for SduI, 10X
10mM Tris-HCl (pH 7.2), 3mM MgCl2, 150mM NaCl and 0.1mg/ml BSA. Incubate at 37C

Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.

Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with SduI (see Star Activity).

Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with SduI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.4uM. More than 95% of these can be recut.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.

Star Activity: A large excess of enzyme (10u/ug DNA x 16 hours), low salt concentration or high pH may result in star activity.

Stability during Prolonged Incubation: A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C.

Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min.

Compatible Ends: Alw21I, ApaI, Eco24I, Mph1103I, PstI, SacI, SdaI

Number of Recognition Sites in DNA:

Lambda: 38
PhiX174: 3
M13mp18/19: 5
pBR322: 10
pUC18/19: 5
pUC57: 6
pTZ19R/U: 5
pBluescriptIIKS(-/+): 6
pBluescriptIISK(-/+): 6
pACYC177: 4
pACYC184: 8

Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.