Technical Data
S1001-15A
Serotonin Receptor 1A (5-HT1A)
Description:
The serotonin receptors, also known as 5-hydroxytryptamine receptors or 5-HT receptors, are a group of G protein-coupled receptors (GPCRs) and ligand-gated ion channels (LGICs) found in the central and peripheral nervous systems. They mediate both excitatory and inhibitory neurotransmission. The serotonin receptors are activated by the neurotransmitter serotonin, which acts as their natural ligand.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: Using rat brain extracts of cortex, hypothalamus, midbrain and hindbrain, the antibody specifically labels a single band. Immunolabeling is abolished by pre-adsorption with synthetic rat 5HT transporter (aa579-599).
Immunohistochemistry (indirect IF): 1:200-1:400
Immunohistochemistry (biotin/streptavidin HRP technique): 1:200-1:600 in rat raphe nuclei, hypothalamus, cortex and spinal cord.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceRatRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to aa294-312 of the rat 5-HT1A receptor coupled to bovine thyroglobulin with glutaraldehyde.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid, 1% BSA.
Specificity:
Recognizes rat Serotonin Receptor 1A. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Burnet, P.W., et al., Brain Research 676(1): 157-168 (1995). 2. Chalmer, D.T. & Watson, S.J., Brain Research 561: 51-60 (1991). 3. Kia, H.K., et al., J. Comp. Neurology 365a(2) 289-305 (1996). 4. Kongins, P.N., et al., J. Receptor & Signal Transduction Research 15(1-4): 443-455 (1995). 5. Raymond, J.R., et al., Am. J. Physiol. 264: F9-F19 (1993). 6. Wright, D.E., et al., J. Comp. Neurol. 351: 357-373 (1995).