Technical Data
S1001-15C
Serotonin Receptor 1A
Description:
Serotonin Receptor 1A, also known as 5-hydroxytryptamine receptor 1A, is a member of the G protein-coupled receptor family. Serotonin Receptor 1A has been found to be expressed highly in the septum and hippocampus. Serotonin Receptor 1A seems to have an inhibitory effect on Ca2+ channels, via N-channels and L-channels. The receptor actuates voltage-sensitive slowing using the N-channels and actuates steady state inhibition using the L-channels.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml detects Serotonin Receptor 1A on 10ug of rat brain tissue lysate. Band is observed at ~40kD. Calculated MW is 46kD.
Immunohistochemistry: 1:400 detects Serotonin Receptor 1A in normal human brain tissue.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM,k11C254Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Linear peptide corresponding to rat Serotonin Receptor 1A, conjugated to KLH ( NP_036717).
Purity:
Purified by ion exchange chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes rat Seratonin receptor 1A. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Albert, P.R., et al., J. Biol. Chem. 265(10): 5825-5832 (1990). 2. Cardenas, C.G., et al., J. Neurophys. 77(6): 3284-3296 (1997).