Technical Data
Serotonin Receptor 3A (5-HT3A, HTR3A, 5-Hydroxytryptamine (Serotonin) Receptor 3A, 5-HT-3, 5-HT3R, HTR3, 5HT3 Serotonin Receptor, Serotonin-gated Ion Channel Receptor)
HT receptors interact with the neurotransmitter and peripheral signal mediator serotonin. In particular, 5-hydroxytryptamine (5HT) receptors mediate the role of serotonin as an endogenous ligand and in pharmaceutical and hallucinogenic drugs. In addition, 5HT receptors affect the release and activities of other neurotransmitters, such as glutamate, dopamine, and GABA. 5HT3A receptor belongs to the serotonin receptor family and is one member of G protein coupled receptor (GPCR) family. The receptor is a ligand-gated ion channel that mediates a fast excitatory response. This receptor is also a member of the ligand-gated ion channel superfamily and is related to the nicotinic, GABAA, and NMDA receptors.

Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Flow Cytometry: 2-5ug for 1x10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
40ug-20CBlue IceHumanRabbit
~0.5mg/ml (after reconstitution)
Synthetic peptide corresponding to the first extracellular domain of human 5-HT3A, conjugated to KLH (Swiss Prot: P46098).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Recognizes endogenous levels of human 5HT3A protein. Species crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.