Technical Data
Serotonin Receptor 4 (HTR4, 5-hydroxytryptamine Receptor 4, 5-HT-4, 5-HT4)
5HT4R, is an ~45kD multipass serotonin receptor that is expressed in the brain, gastrointestinal tract, bladder, vascular endothelial cells, atrium, kidney, and adrenal gland. It is involved in the regulation of learning, memory, anxiety, smooth muscle contraction, and adrenal corticosteroid secretion. Alternate splicing of human 5HT4R generates multiple isoforms with either a 14aa insertion in the second extracellular loop or a deletion or various substitutions in the C-terminal cytoplasmic region. Different isoforms show tissue specific expression patterns or increased activity in the absence of ligand. Human 5HT4R shares 94-88aa sequence identity with mouse and rat 5HT4R, respectively.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2B11n173Affinity Purified
100ug-20CBlue IceHumanMouse
Not determined
Recombinant corresponding to human HTR4 expressed in NSO cells.
Purified by Protein A/G affinity chromatography.
Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Recognizes human HTR4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.