Technical Data
Serum Amyloid A4, Constitutive (SAA4, CSAA)
Serum amyloid A4 (SAA4) is a constitutively expressed SAA protein that is also known as C-SAA. It is an apolipoprotein that constitutes more than 90% of SAA on HDL during homeostasis and may be involved in lipid transfer between lipoprotein classes. Mature human SAA4 contains 112 amino acid (aa) residues. It is primarily produced in hepatocytes and secreted plasma. In solution, SAA4 is reported to form stable dimers and trimers. Human and mouse SAA4 share 57% aa sequence identity.

Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1ug/ml
Immunohistochemistry: 5-15ug/ml will detect Serum Amyloid A4 in cells and tissues. Requires HEIR using Tris-EDTA, pH 9.0.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
~0.2mg/ml (after reconstitution)
Recombinant protein corresponding to aa19-130 of human Serum Amyloid A4 (P35542) expressed in E. coli.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Recognizes human Serum Amyloid A4. Species Crossreactivity: recombinant mouse SAA4 (<20%). Shows <5% crossreactivity with recombinant human SAA1 and recombinant human SAA2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.