Technical Data
S1002-60A
Serum Amyloid A4, Constitutive (SAA4, CSAA)
Description:
Serum amyloid A4 (SAA4) is a constitutively expressed SAA protein that is also known as C-SAA. It is an apolipoprotein that constitutes more than 90% of SAA on HDL during homeostasis and may be involved in lipid transfer between lipoprotein classes. Mature human SAA4 contains 112 amino acid (aa) residues. It is primarily produced in hepatocytes and secreted plasma. In solution, SAA4 is reported to form stable dimers and trimers. Human and mouse SAA4 share 57% aa sequence identity.

Applications:
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml; The detection limit for recombinant human SAA4 is ~0.1ng/well.
Western Blot: 0.1-0.2ug/ml; The detection limit for recombinant human SAA4 is ~0.5ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 2-15ug/ml will detect SAA4 in cells and tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa19-130 of human SAA4 (P35542) expressed in E. coli.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:
Recognizes human SAA4. Species Crossreactivity: recombinant mouse SAA4 (~20%). Shows <5% crossreactivity with recombinant human SAA1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.