Technical Data
SHC (Tyr427) (SHC-transforming Protein 1, SHC-transforming Protein 3, Src Homology 2 Domain-containing-transforming Protein C1, SH2 Domain Protein C1, SHC-transforming Protein A, SHC1, SHCA)
Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:10-1:50
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to a region within aa413-443, surrounding Y427 of human SHC, conjugated to KLH. Species Sequence Homology: bovine
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human SHC.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pelicci G., Cell 70:93-104(1992). 2. Craparo A., J. Biol. Chem. 270:15639-15643(1995).