Technical Data
S1012-99M
Shh-a, ID (Sonic Hedgehog Protein A, SHHA, VHH-1, shh)
Description:
Sonic hedgehog a (shha) is a member of the hedgehog gene family. The hedgehog family of genes specifies the fate of a large number of different cell types within invertebrate and vertebrate embryos. Genetic analyses of the zebrafish orthologue of mammalian scube-2 indicates its involvement in hedgehog (Hh) signaling. Duplication events have occurred evolutionarily in teleost fish with regard to sonic hedgehog and indian hedgehog. Formation of the adenohypophysis in zebrafish is largely dependent on shha, as in mice, demonstrating highly conserved function. Hedgehog is essential to adult hematopoietic stem cell formation in zebrafish.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceZebrafishRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the intermediate region of zebrafish Shha.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes zebrafish Shha.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hollway, GE et al (2006) Dev. Biol. 294(1):104-118. 2. Ingham, PW and Kim, HR (2005) Exp. Cell Res. 306(2):336-342. 3. Herzog, W et al (2003) Dev. Biol. 254(1):36-49. 4. Gering, M and Patient, R. (2005) Dev. Cell 8(3):389-400.