Description:
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 1:4000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 5i84 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 50ug | | 4°C (-20°C Glycerol) | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~0.5mg/ml |
| Immunogen: |
| Ovalbumin-conjugated synthetic peptide corresponding to N-terminal amino acids 1-50 of human silent information regulator 2 homolog (Sirt7). |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 40% glycerol. |
| Specificity: |
| Recognizes human Sirt7, a NAD-dependent deacetylase, cytoplasmatic protein located under the nuclear membrane involved in diverse processes ranging from regulation of gene silencing to DNA damage response in mammalian cells. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Smith, J.S. et. al. (2002) Methods Enzymol. 353, 282 - 300.0 2. Frye, R. (2000) Biochem. Biophys. Res. Commun. 273, 793 - 798. 3. Frye, R. (1999) Biochem. Biophys. Res. Commun. 260, 273 - 279.
|
|
