Technical Data
SLP 76 (SH2-domain Leukocyte Protein)
SLP 76 was recently identified as a substrate of the T cell receptor stimulated protein tyrosine kinase. This molecule is rapidly phosphorylated on T cell receptor activation. SLP 76 has three identifiable domains; the first containing a number of tyrosines which are the likely sites of phosphorylation, the second a proline-rich domain, and the third an SH2 domain. SLP 76 has been shown to bind to the Grb2 adapter protein in vivo; the association between Grb2 and SLP 76 has been mapped to a 20 amino acid stretch in SLP76's proline-rich region. The SH2 domain of SLP 76 binds to two tyrosine phosphorylated proteins after T cell receptor activation. These molecules are yet to be identified. The SH2 domain of SLP 76 also appears to bind to a serine threonine kinase, perhaps via an indirect association.. Interestingly, overexpression of SLP 76 results in greatly augmented IL-2 production in transiently transfected T cells. Collectively, these data suggest that SLP 76 plays an important role in T cell activation and perhaps is involved in coupling signaling pathways after activation of the T cell antigen receptor. Immunogen: Bacterially expressed GST-SLP 76 fusion protein translated from the cloned human cDNA.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml.. Detects SLP 76 in RIPA lysates from Jurkat cells.
Immunoprecipitation: 4ug from a previous lot immunoprecipitated SLP 76 from 500ug of Jurkat cell RIPA lysate. Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
J9000: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
250ug-20CBlue IceHumanSheep
Bacterially expressed GST-SLP 76 fusion protein.
Purified by Protein G affinity chromatography.
Supplied as a solution in 250ul of 0.1M Tris-glycine, pH 7.5, 150mM sodium chloride, 0.05% sodium azide.
Recognizes human SLP 76 at ~76kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Jackman, J.K., et al, J. Biol. Chem., 270: 7029, 1995.