Technical Data
S1014-20D
SLP 76 (SH2-domain Leukocyte Protein)
Description:
SLP 76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through Grb2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP 76 include phospholipase C (PLC)–gamma, Vav, Nck and fyn binding protein.

SLP 76 was recently identified as a substrate of the T cell receptor stimulated protein tyrosine kinase. This molecule is rapidly phosphorylated on T cell receptor activation. SLP 76 has three identifiable domains; the first containing a number of tyrosines which are the likely sites of phosphorylation, the second a proline-rich domain, and the third an SH2 domain. SLP 76 has been shown to bind to the Grb2 adapter protein in vivo; the association between Grb2 and SLP 76 has been mapped to a 20 amino acid stretch in SLP76's proline-rich region. The SH2 domain of SLP 76 binds to two tyrosine phosphorylated proteins after T cell receptor activation. These molecules are yet to be identified. The SH2 domain of SLP 76 also appears to bind to a serine threonine kinase, perhaps via an indirect association.. Interestingly, overexpression of SLP 76 results in greatly augmented IL-2 production in transiently transfected T cells. Collectively, these data suggest that SLP 76 plays an important role in T cell activation and perhaps is involved in coupling signaling pathways after activation of the T cell antigen receptor. Immunogen: Bacterially expressed GST-SLP 76 fusion protein translated from the cloned human cDNA.

Applications:
Suitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested.

Recommended Dilution:
Western Blot: 1:500, detects a band of approximately 76kD in T lymphocyte cell lysates.
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:HRP

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b6A462Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
A fusion protein corresponding to amino acids 21.6-434 of human SLP-76.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes the SH2 domain-containing leucocyte protein (SLP-76), a 76kD tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage. Species Cross Reactivity: Human, Mouse, Porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bonilla, F. A. et al. (2000). Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fc gamma receptors I and II/III. PNAS 97(4):1725-1730 2. Pivniouk, V.I. et al. (1999). SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast cells. J. Clin. Invest. 103(12):1737-1743 3. Delaguillaumie, A. et al. (2002). Rho GTPases link cytoskeletal rearrangements and activation processes induced via the tetraspanin CD82 in T lymphocytes. J Cell Science 115:433-443.