Technical Data
S1014-20S
SLP-76, NT (SH2 Domain Containing Leukocyte Protein 76 kD, SH2 Domain-containing Leukocyte Protein of 76kD, SH2 Domain Containing Leukocyte Protein of 76kDa, SLP76, SLP76 Tyrosine Phosphoprotein, SLP-76 Tyrosine Phosphoprotein, 76kD Tyrosine Phosphoprotei
Description:
SH2 domain-containing leukocyte protein (SLP-76) is a 76kD tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage. SLP-76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through GRB2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP-76 include phospholipase C (PLC)-gamma, Vav, Nck and fyn binding protein.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide sequence C-ALRNVPFRSEV corresponding to the N-terminus of SLP-76 (NP_005556).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Specificity:
Recognizes human SLP-76 at ~75kD in Jurkat cell lysates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.