Technical Data
S1014-25
Sm (Smith) Antigen
Description:
The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.

Applications:
Suitable for use in ELISA, Immunodiffusion and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
2ml4°C (-20°C Glycerol)Blue IceHumanHuman
Concentration:
Not determined.
Immunogen:
Sm(Smith). Autoantibody derived from human serum.
Purity:
Purified by fractionation of delipidated and defibrinated donor human serum.
Form
Supplied as a lyophilized powder in 0.01 M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes human Sm. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Crossreactivity: RNP and anti-SSA (low levels). No reactivity at a dilution of 1:100 when tested against purified SSB, Scl-70 and Jo-1 in an ELISA system designed to detect all IgG subclasses.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.