Technical Data
Smad1 (MADR1, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Smad 1 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad 1 is involved in cell growth, apoptosis, morphogenesis, development and immune response (2). After phosphorylaion by the BMP type 1 receptor kinase Smad1 and co-Smad4 form a heteromeric complex and translocate to the nucleus to regulate transcription of target genes (3).

Suitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:10-1:50
Immunocytochemistry: 1:10-1:50

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul -20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues in human Smad1.
Supplied as a liquid.
Recognizes human Smad1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wrana, J. L. 2000. Regulation of Smad activity. Cell 100:189-192.
2. Massague, J. 2000. How cells read TGF-beta signals. Nat. Rev. Mol. Cell Biol. 1:169-178.
3. Wrana et al. Nature 389:631-635.