Technical Data
S1014-51F
Smad1 (MADR1, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
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Technical Data |
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S1014-51F |
Smad1 (MADR1, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) |
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Description: Smad 1 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad 1 is involved in cell growth, apoptosis, morphogenesis, development and immune response (2). After phosphorylaion by the BMP type 1 receptor kinase Smad1 and co-Smad4 form a heteromeric complex and translocate to the nucleus to regulate transcription of target genes (3). Applications: Suitable for use in Western Blotting, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blotting: 1:10-1:50 Immunocytochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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