Technical Data
Smad1, phosphorylated (Ser465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), are downstream effectors of TGFb/BMP signaling. Smad1 (MADR1) mediates signals of bone morphogenetic proteins (BMPs) in the cell. BMPs constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. BMP receptors are members of the TGFb family of
Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation and activation of these receptors. Subsequently, they phosphorylate Smad1 at serine 463 and 465 in the carboxyl-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the collaborating Smad4 (DPC4) and are translocated into the nucleus, where the transcription of target genes is stimulated.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthesized phosphopeptide derived from human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS (Mg2+ and Ca2+ free), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of human Smad1 only when phosphorylated at serine 465. Species Crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.