		Technical Data
S1014-53A3	SMAD2, 3 (Mothers Against Decapentaplegic Homolog 2, MAD Homolog 2, Mothers Against DPP Homolog 2, JV18-1, Mad-related Protein 2, hMAD-2, SMAD Family Member 2, SMAD 2, MADH2, MADR2/Mothers Against Decapentaplegic Homolog 3, MAD Homolog 3, Mad3, Mothers Ag

Description:
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-b signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Chromatin IP, Chip Aragose and Chip Magnetic. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IF-IC): 1:125
Flow Cytometry: 1:200
Western Blot: 1:1000
Immunoprecipitation: 1:50
Chromatin IP: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to residues surrounding Ala208 of human Smad2.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Specificity:

Recognizes endogenous levels of total human Smad2/3. Species Crossreactivity: Monkey, mouse, Xenopus and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Heldin, C.H. et al. (1997) Nature 390, 465-471. 2.Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194. 3.Derynck, R. et al. (1998) Cell 95, 737-740. 4.Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791. 5.Whitman, M. (1998) Genes Dev. 12, 2445-2462. 6.Wu, G. et al. (2000) Science 287, 92-97. 7.Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-1647. 8.Moustakas, A. et al. (2001) J. Cell Sci. 114, 4359-4369.