Technical Data
S1014-53A3
SMAD2, 3 (Mothers Against Decapentaplegic Homolog 2, MAD Homolog 2, Mothers Against DPP Homolog 2, JV18-1, Mad-related Protein 2, hMAD-2, SMAD Family Member 2, SMAD 2, MADH2, MADR2/Mothers Against Decapentaplegic Homolog 3, MAD Homolog 3, Mad3, Mothers Ag
Description:
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-b signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Chromatin IP, Chip Aragose and Chip Magnetic. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IF-IC): 1:125
Flow Cytometry: 1:200
Western Blot: 1:1000
Immunoprecipitation: 1:50
Chromatin IP: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Ala208 of human Smad2.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human Smad2/3. Species Crossreactivity: Monkey, mouse, Xenopus and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Heldin, C.H. et al. (1997) Nature 390, 465-471. 2.Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194. 3.Derynck, R. et al. (1998) Cell 95, 737-740. 4.Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791. 5.Whitman, M. (1998) Genes Dev. 12, 2445-2462. 6.Wu, G. et al. (2000) Science 287, 92-97. 7.Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-1647. 8.Moustakas, A. et al. (2001) J. Cell Sci. 114, 4359-4369.