Technical Data
SMAD2, 3 (Mothers Against Decapentaplegic Homolog 2, MAD Homolog 2, Mothers Against DPP Homolog 2, JV18-1, Mad-related Protein 2, hMAD-2, SMAD Family Member 2, SMAD 2, MADH2, MADR2/Mothers Against Decapentaplegic Homolog 3, MAD Homolog 3, Mad3, Mothers Ag
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-b signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

Suitable for use in Immunofluorescence/ Immunocytochemistry, Flow Cytometry, Western Blot, Immunoprecipitation, Chromatin IP, Chip Aragose and Chip Magnetic. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IF-IC): 1:125
Flow Cytometry: 1:200
Western Blot: 1:1000
Immunoprecipitation: 1:50
Chromatin IP: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues surrounding Ala208 of human Smad2. Species sequence homology: Xenopus (100%)
Purified by Protein A affinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Recognizes endogenous levels of total human Smad2/3. Species Crossreactivity: Monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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