Technical Data
SMAD3 (Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, SMAD Family Member 3, SMAD 3, HSPC193, HsT17436, JV15-2, MADH3, MGC60396)
SMAD3, a member of SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain.It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa100-150 of human SMAD3.
Purified by Protein G affinity Chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human SMAD3. Species Crossreactivity: bovine, canine, mouse, porcine, rat, rhesus monkey. Species sequence homology: zebrafish, xenoupus, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Qin Genes & Development 16 (15); 1950-1963 (August 1, 2002) 2. Shi Cell 113 (6); 685-700 (13 June, 2003)