Technical Data
S1014-54E1
SMAD3, phosphorylated (Ser208) (Mothers Against Decapentaplegic Homolog 3, SMAD 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, hMAD-3, SMAD Family Member 3, JV15-2, hSMAD3, MADH3)
Description:
SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

Applications:
Suitable for use in ELISA, Dot Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser208 of human SMAD3 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human SMAD3 when phosphorylated at Ser208.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Imoto, S., et al., FEBS Lett. 579(13):2853-2862 (2005). 2. Dubrovska, A., et al., Oncogene 24(14):2289-2297 (2005). 3. Furumatsu, T., et al., J. Biol. Chem. 280(9):8343-8350 (2005). 4. Kobayashi, T., et al., Biochem. Biophys. Res. Commun. 327(2):393-398 (2005). 5. Kamaraju, A.K., et al., J. Biol. Chem. 280(2):1024-1036 (2005).