		Technical Data
S1014-55C5	SMAD4 (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4)

Description:
DPC4 (also named SMAD4; small mothers against decapentaplegic deleted in pancreatic carcinoma). The family of SMAD molecules comprises essential mediators of transforming growth factor beta (TGF-b) signaling. To date, seven members of this family have been identified, each plays a specific and separate role in mediating TGF-b superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well.

Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2h at RT
Immunoprecipitation: 2ug/mg protein lysate
Optimal dilutions to be determined by the researcher.

Positive Control:
LS174T, HT29, raji, jurkat cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	2D79	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Purified recombinant DPC4.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

Specificity:

Recognizes human SMAD4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.