Technical Data
SMAD 1/5/8/9 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma)
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. For additional information on SMAD proteins, please follow the provided links to the NCBI website: human SMAD1, SMAD2, SMAD3, SMAD4, SMAD5, SMAD6, SMAD7, SMAD9 and mouse SMAD8.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive control: A549 whole cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptides corresponding to amino acids 18-32, LGWKQGDEEEKWAEK, and 315-329, SNVNRNSTIENTRRH of human SMAD1. Species sequence homology: chicken, chimpanzee, invertebrates, mammals, porcine, Xenopus and zebrafish.
Purified by Protein G chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes human SMAD 1/5/8/9. Species Crossreactivity: mouse, rat.. May cross-react with SMAD 2 and 3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kawai,S., Faucheu,C., Gallea,S., Spinella-Jaegle,S., Atfi,A., Baron,R. and Roman,S.R. Mouse smad8 phosphorylation downstream of BMP receptors ALK-2, ALK-3, and ALK-6 induces its association with Smad4 and transcriptional activity. Biochem. Biophys. Res. Commun. 271 (3), 682-687 (2000)