Technical Data
S1014-97B2
SNX2 (Sorting Nexin 2, Sorting Nexin-2, MGC5204, Transformation-related Gene 9 Protein, TRG9, TRG-9)
Description:
Snx2 is a signaling protein belonging to the sorting nexin family and characterized by the presence of a 100aa region known as the phox (PX) homology domain (a phosphoinositide binding domain). As a transport carrier Snx2 is involved in several stages of intracellular trafficking of proteins and also in the EGFR signaling pathway. The retromer complex that is composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, Snx1 and Snx2, is required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Though Snx2 associates with formin-binding protein 17, its function is yet unknown. It may form oligomeric complexes with itself as well as with other family members Snx1, Snx1A, and Snx4, but not with Snx3 and is ubiquitously expressed in most tissues, including peripheral leukocytes.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse Placenta lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa350-400 of human Snx2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Snx2. Species Crossreactivity: chimpanzee, bovine, canine, mouse, opossum. Species sequence homology: equine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rojas, R. et al. Mol. Cell Biol. 27:1112-1124 (2007). 2. Carlton, JG. et al. J. Cell. Sci. 118:4527-4539 (2005). 3. Haft, CR. et al. Molec. Cell. Biol. 18:7278-7287 (1998).