Technical Data
S1014-97J
SNX15 (Sorting Nexin 15)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.2-1.0ug/ml. By Western blot, this antibody detects a band of 40-45kD in 293 and human kidney cell lysate, which corresponds to the predicted molecular weight of SNX 15 (predicted MW 40kD according to NP_037438).
A single band (approx. 30-35kD, probably representing the shorter isoform) was observed in human heart lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
293 or human kidney cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide with sequence EEILRLHLSQLPP, from C Terminus of the protein sequence according to NP_037438; NP_680086.
Purity:
Purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form
Supplied as a liquid in tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Expected to recognize both human isoforms. Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Phillips SA et al. Identification and characterization of SNX15, a novel sorting nexin involved in protein trafficking. J Biol Chem 276 :5074-84 (2001). PubMed: 11085978.