Technical Data
S1040-01C
SOCS 1 (Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1)
Description:
SOCS (Suppressor of Cytokines Signaling) proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.
SOCS 1, also called Janus kinase (JAK) binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is an SH2-domain-containing protein. It was shown to interact with all four Jak kinases (Jak1, Jak2, Jak3 and Tyk2) and is induced by and inhibits the signaling action of IL-3, IL-4, IL-6, growth hormone, LIF, prolactin, interferon gamma (IFNg), and erythropoietin (2). It plays an important role in the negative regulation of IFNg signaling and in the differentiation of T cells
(3). SOCS 1 mRNA is highly expressed in the thymus, to a much lower level in the spleen, and not expressed in most other tissues (4). The SOCS 1 protein is primarily expressed in the thymocytes independent of the cytokine and T cell receptor (5). Biochemical characterization as well as gene disruption studies indicate that SOCS 1 is an important negative regulator of the JAK-STAT signal pathway.

Applications:
Suitable for use in ELISA (immunogen only), Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml
Immunoprecipitation: 1-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k4H314Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein derived from the N-terminal region of mouse SOCS 1. Species Sequence Homology: human, rat; 95%.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse SOCS 1, NT at ~30kD. Reactivity confirmed with mouse thymus, spleen, lung and liver homogenates as well as with mouse recombinant protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang, J-G., et al., PNAS 98(23): 13261-13265 (2001). 2. Sporri, B., et al., Blood 97: 221-226 (2001). 3. Krebs, D.L. and Hilton, D.J., J. Cell. Sci. 113: 2813-2819 (2000). 4. Marine, J-C., et al., Cell 98: 609-616 (1999). 5. Blumenstein, M., et al., J. Clin. Endocrinol. Metab. 87(3): 1094-1097 (2002)