Technical Data
S1040-01D
SOCS 1 (Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1)
Description:
The suppressor of cytokine signaling (SOCS) family of proteins (SOCS1-SOCS7 and CIS) inhibit cytokine production by a classical negative feedback mechanism. These proteins are upregulated in response to cytokine stimulation followed by inhibition of cytokine-inducing signal pathway. Role of each members of the family has been determined by gene knockout experiments (1). SOCS1 also known as JAB and (STAT)-induced STAT inhibitor 1 (SSI-1) is an SH2-domain-containing protein that is induced by and negatively regulates signaling by a number of cytokines including interleukin-4 (IL-4), IL-6, interferon (IFN)-gamma, prolactin, growth hormone, and erythropoietin (1). SOCS1/JAB is rapidly induced by LPS and negatively regulates LPS signaling (2). SOCS1 (-/-) mice are very sensitive to LPS. These mice die prematurely, probably due to uncontrolled IFN-g signaling (3).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

positive control:
Human Ramos or mouse Raw cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide (HFRTFRSHADYRRIT) corresponding to amino acids 54-68 of human SOCS1, GenBank NP_003736.1
Purity:
Purified by Protein G chromatography
Form
Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.02% sodium azide.
Specificity:
Recognizes human SOCS 1 (Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1). Species Crossreactivity: Mouse and human. Species sequence homology: Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krebs DL, Hilton DJ. Stem Cells 19(5):378-387 (2001).

2. Kinjyo I, Hanada T, Inagaki-Ohara K, Mori H, et al. Immunity 17(5):583-591 (2002).

3. Ilangumaran S, Finan D, La Rose J, Raine J, Silverstein A, De Sepulveda P, Rottapel R. J Immunol 169(9):5010-5020 (2002).