Technical Data
SOCS 1(Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1)
SOCS-1, also called Janus kinase (JAK) binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is an SH2-domaincontaining protein. It was shown to interact with all four Jak kinases (Jak1, Jak2, Jak3 and Tyk2) and is induced by and inhibits the signaling action of IL-3, IL-4, IL-6, growth hormone, LIF, prolactin, interferon gamma (IFN-g), and erythropoietin. It plays an important role in the negative regulation of IFN-g signaling and in the differentiation of T-cells. SOCS-1 mRNA is highly expressed in the thymus, to a much lower level in spleen, and not expressed in most other tissues. Biochemical characterization as well as gene disruption studies indicate that SOCS-1 is an important negative regulator of the JAK-STAT signal pathway.

Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Controls:
Mouse thymus cell lysates and HepG2 human hepatocellular carcinoma lysate.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C (-20C Glycerol)Blue IceHumanRabbit
A synthetic peptide derived from the C-terminal region of human, mouse or rat SOCS-1 protein
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Recognizes the human SOCS-1 protein at ~30kD. Species Crossreactivity: mouse Species Sequence Homology: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sporri B, et al. Blood 97: 221-226, 2001. 2. Krebs D.L., Hilton, D.J. J Cell Sci. 113: 2813-2819, 2000. Marine JC, et al. Cell 98: 609-616, 1999.