Technical Data
S5380-05
Spectrin, alpha II, SH3 domain, non-erythroid (Fodrin
Description:
This antibody was developed against the alpha II spectrin SH3 domain.(1) Specific marker for human, rat and mouse alpha II spectrin or fodrin.

Source: Tissue culture supernatant

Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry (Frozen only). Other applications not tested.

Recommended Dilution: Immunofluorescence: 1:100-1:500, Immunohistochemistry: 1:50-1:100; Optimal dilutions to be determined by the researcher.

Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b2B81Highly Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain
Purity:
Purified by ammonium sulfate precipitation and dialyzed.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin. Does not crossreact with alpha I (erythroid) spectrin SH3 domain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Xu, J., et al., "Monoclonal antibodies to alpha I spectrin Src homology domain 3 associate with macropinocytic vesicles in nonerythroid cells", Brain Research, 898: 171-177 (2001).
2. Xu, J., et al., "Human spectrin SH3 domain binding protein I regulates macropinocytosis in NIH 3T3 cells", J. Cell Science, 113: 3805-3814 (2000).
3. De Matteis, M.A., et al., "Spectrin tethers and mesh in the biosynthetic pathway", J. Cell Science, 113: 2331-2343 (2000).
4. Swanson, J.A., et al., "Macropinosytosis Trends", Cell Biol., 5: 424-427 (1995)
5. Lamaze, C., et al., "The emergence of clathrin-independent pinocytic pathway", Curr. Opin. Cell Biol., 7: 573-580 (1995).
6. Bennett, V., et al., "The spectrin-based membrane skeleton and micron scale organization of the plasma membrane", Annu. Rev. Cell Biol., 9: 27-66 (1993).