Technical Data
S5380-20
Spectrin Binding Protein, SH3 domain, Hssh3bp1 (SBP)
Description:
This antibody was developed against the spectrin SH3 domain binding protein Hssh3bp1.(3) Hssh3bp1 is a marker of macropinocytic vesicles and a potential regulator of macropinocytosis.(2) The gene encoding Hssh3bp1 has a role as a tumor suppressor gene which is inactivated during prostate tumorigenesis.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:2000
Immunofluorescence: 1:100-1:500
Immunohistochemistry (FFPE and frozen sections): 1:40-1:100 Requires pretreatment for antigen unmasking.
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG2b2B82Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant Hssh3bp1 (human spectrin SH3 domain binding protein 1)
Purity:
Purified by Protein A chromatography.
Form
Supplied as a liquid in PBS. No preservatives added.
Specificity:
Recognizes human spectrin SH3 domain binding protein 1. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Macoska, J.A., et al., Neoplasia., 3: 1-6 (2001) 2. Xu, J., et al., J. Cell Science, 113: 3805-3814 (2000). 3. Ziemnicka-Kotula, D., et al., J. Biol. Chem., 273: 13681-13692 (1998) 4. Bennett, V., et al., Annu. Rev. Cell Biol., 9: 27-66 (1993) 5. De Matteis, M.A., et al., J. Cell Science, 113: 2331-2343 (2000). 6. Lamaze, C., et al., Curr. Opin. Cell Biol., 7: 573-580 (1995). 7. Swanson, J.A., et al., Cell Biol., 5: 424-427 (1995).