		Technical Data
S5381-03B	Spectrin (Alpha I Spectrin, Alpha-Spec, Beta I Spectrin, Beta Spectrin, Beta-Spec, Elliptocytosis 2, Elliptocytosis-2, EL2, Erythrocyte alpha Spectrin, Erythroid Alpha Spectrin, HSpTB1, Membrane Cytoskeletal Protein, Spec B, Spectrin alpha Chain, Spec A,

Description:
Spectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head to head self association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2 dimensional network.

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:40. Detects a band at 220kD and 240kD
Immunofluorescence: 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Chicken	Rabbit

Concentration:

Not Determined

Immunogen:

Full length, purified, native spectrin alpha and beta chain from chicken. Cellular Localization: Cytoplasmic surface of the cell membrane.

Purity:

Serum

Form

Supplied as a liquid, 0.1% sodium azide.

Specificity:

Recognizes chicken Spectrin. Species Crossreactivity: Crossreacts with a wide range of avian and mammalian species.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.