Technical Data
S5381-03B
Spectrin (Alpha I Spectrin, Alpha-Spec, Beta I Spectrin, Beta Spectrin, Beta-Spec, Elliptocytosis 2, Elliptocytosis-2, EL2, Erythrocyte alpha Spectrin, Erythroid Alpha Spectrin, HSpTB1, Membrane Cytoskeletal Protein, Spec B, Spectrin alpha Chain, Spec A,
Description:
Spectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head to head self association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2 dimensional network.

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:40. Detects a band at 220kD and 240kD
Immunofluorescence: 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
200ul-20CBlue IceChickenRabbit
Concentration:
Not Determined
Immunogen:
Full length, purified, native spectrin alpha and beta chain from chicken. Cellular Localization: Cytoplasmic surface of the cell membrane.
Purity:
Serum
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes chicken Spectrin. Species Crossreactivity: Crossreacts with a wide range of avian and mammalian species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.