Technical Data
S5381-03E
Spectrin, alpha (Spectrin Non-erythroid alpha Chain, Alpha Fodrin, Alpha II Spectrin, FLJ44613, Spectrin alpha Non-erythrocytic 1, SPTAN1, SPTA2)
Description:
The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1AA6Ascites
SizeStorageShippingSourceHost
100ug-20°CBlue IceChickenMouse
Concentration:
~0.1mg/ml
Immunogen:
Chicken red blood cell membranes
Purity:
Ascites
Form
Supplied as a liquid in PBS, 1% BSA, 0.1 % sodium azide.
Specificity:
Recognizes chicken a-spectrin at ~240kD. Reacts with the a-spectrin molecule of all mammalian nonerythroid cells. Species Crossreactivity: human, rat, rabbit, guinea pig.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sormunen R. et al. (1999) J. Pathol. 187, 416-423. 2. Vääräniemi J. et al. (1997) Eur. J. Cell Biol. 74, 262-272. 3. Tuominen H. et al. (1996) Br. J. Dermatol. 135, 576-580. 4. Huotari V. et al. (1996) J. Cell. Physiol. 167, 121-130. 5. Sormunen R. et al. (1994) Am. J. Resp. Cell Mol. Biol. 11, 75-84. 6. Vääräniemi J. et al. (1994) Biochim. Biophys. Acta 1189, 21-30. 7. Sormunen R. et al. (1993) Histochem. J. 25, 678-686. 8. Sormunen R. et al. (1993) Lab. Invest. 68, 652-662. 9. Reima I. et al. (1993) Differentiation 54, 35-45. 10. Eskelinen S. et al. (1992) J. Cell. Physiol. 150, 122-133. 11. Huotari V. et al. (1992) J. Cell. Physiol. 153, 340-352. 12. Ylikoski J. et al. (1990) Hearing Res. 43, 199-204.