		Technical Data
S5381-03E	Spectrin, alpha (Spectrin Non-erythroid alpha Chain, Alpha Fodrin, Alpha II Spectrin, FLJ44613, Spectrin alpha Non-erythrocytic 1, SPTAN1, SPTA2)

Description:
The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	AA6	Ascites

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Chicken	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Chicken red blood cell membranes

Purity:

Ascites

Form

Supplied as a liquid in PBS, 1% BSA, 0.1 % sodium azide.

Specificity:

Recognizes chicken a-spectrin at ~240kD. Reacts with the a-spectrin molecule of all mammalian nonerythroid cells. Species Crossreactivity: human, rat, rabbit, guinea pig.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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