Technical Data
S5381-04A
Sptan1 (Spectrin alpha Chain, Non-erythrocytic 1, Alpha-II Spectrin, Fodrin alpha Chain, Spna2, Spta2)
Description:
Fodrin is a universally expressed membrane-associated cytoskeletal protein consisting of a- and b-subunits. The protein is important for maintaining normal membrane structure and supporting cell surface protein function. a-Fodrin is one of the important targets cleaved by caspases during apoptosis. The full length 240kD protein can be cleaved at several sites within its sequence by activated caspases generating N-terminal 150kD, C-terminal 120kD, and 35kD major products. Cleavage of a-Fodrin leads to membrane malfunction and cell shrinkage

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry: 10-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa162 from mouse alpha-Fodrin.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes mouse alpha-Fodrin. Species Crossreactivity: human, rat and chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.