Technical Data
Sprouty 4 (Sprouty-4, Sprouty Homolog 4, Spry 4, SPRY4, SPRY-4)
Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Cellular Localization: Cytoplasmic

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide with sequence CKAASGDAKTSRPDKPF conjugated to KLH, corresponding to amino acids 283-299 of Human Sprouty 4. Epitope: aa 283-299
Purified by immunoaffinity chromatography.
0.15M Sodium Chloride, 0.02M Potassium Phosphate [pH 7.2]
Species Crossreactivity: Expected to crossreact with Human (100% identity with immunogen) and Mouse (88% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Sasaki A et al. Identification of a dominant negative mutant of Sprouty that potentiates fibroblast growth factor- but not epidermal growth factor-induced ERK activation. J Biol Chem 276:36804-8 (2001).