Technical Data
S6500-06E
Src, phosphorylated (Tyr416) (pp60Src, pp60c-Src)
Description:
The Src family of protein tyrosine kinases including Src, Lyn, Fyn, Yes, Lck, Blk, Hck, etc.) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity. Phosphorylation of Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). Lyn is a member of the Src family that is predominantly expressed in hematopoietic cells (3). Lyn participates in signaling from multiple cell surface receptors such as the B cell antigen receptor (BCR) and CD40 (4). Lck is essential for T-lymphocyte activation and differentiation (5,6). Phosphorylation of the carboxy-terminal Tyr505 downregulates Lck activity, while phosphorylation at Tyr394 leads to an increase in Lck activity (7).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul -20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Tyr416 of human Src. Species sequence homology: chicken and xenopus.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of human Src only when phosphorylated at tyrosine 416. The antibody may crossreact with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. It does not crossreact with Src phosphorylated at tyrosine 527. It may crossreact with phosphorylated RTKs. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513609. (2) Hunter, T. (1987) Cell 49, 1-4. (3) Yamanshi,, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 65386542. (4) Yamanashi, Y. et al. (1991) Science 251, 192194. (5) Molina, T.J. et al. (1992) Nature 357, 161164. (6) Straus, D.B. et al. (1992) Cell 70, 585593. (7) Chow, L.M. et al. (1993) Nature 365, 156160.