Technical Data
S7971-06B
STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (Biotin)
Description:
The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators invovled in cytokine signaling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes.
STAT3 is activated by tyrosine phosphorylation at residue Tyr705 in cells treated with Interleukin 6 or EGF. Activated STAT3 can bind to DNA either as a homodimer or as a heterodimer with STAT1.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Immunoprecipitation: 1-10ug per 10e6 pervanadate treated HepG2 cells.
Immunocytochemistry: 1-10ug/ml
ELISA: 0.05ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control Included:
H2030-03H1: HepG2 Cell Lysate, Vanadate Treated

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D76Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa703-707 (A703P[pY]LK707) of STAT3 (KLH). Molecular Weight: 92kD.
Purity:
Purified by subsequent thilliophillic adsorption and size exclusion chromatography.
Form
Supplied as a liquid in PBS, Sucrose, PEG, 0.09% sodium azide. Labeled with Biotin.
Specificity:
Recognizes human STAT3 phosphorylated at Tyr 705. Species Crossreactivity: mouse and canine. Does not crossreact with the non-phosphorylated form of STAT3, nor with unrelated tyrosine-phosphorylated proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.