Technical Data
S7973-30U
Stem Cell Factor, Recombinant, Human (SCF, SF, FPH2, c-Kit Ligand, Kit Ligand, Kitl, KITLG, KL-1, Mast Cell Growth Factor, MGF, SHEP7)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 165aa of human Stem Cell Factor expressed in E.coli is a single, non-glycosylated polypeptide chain.

Molecular Weight:
~18.4kD

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of human TF-1 cellsis less then 2ng/ml, corresponding to a Specific Activity of 105 IU/mg.

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~18.4kD
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Successful mobilization of peripheral blood stem cells using recombinant human stem cell factor in heavily pretreated patients who have failed a previous attempt with a granulocyte colony-stimulating factor-based regimen. Bone Marrow Transplant 2005 Sep;36(5):389-96. 2. Ancestim (recombinant human stem cell factor, SCF) in association with filgrastim does not enhance chemotherapy and/or growth factor-induced peripheral blood progenitor cell (PBPC) mobilization in patients with a prior insufficient PBPC collection. Bone Marrow Transplant 2004 Oct;34(8):683-91. 3. Ex vivo manipulation of umbilical cord blood-derived hematopoietic stem/progenitor cells with recombinant human stem cell factor can up-regulate levels of homing-essential molecules to increase their transmigratory potential. Exp Hematol 2003 Dec;31(12):1237-46. 4. Degranulation and cytokine expression in human cord blood-derived mast cells cultured in serum-free medium with recombinant human stem cell factor. Mol Cells 2003 Oct 31;16(2):154-60. 5. Expression of a novel recombinant dual human stem cell factor in insect cells. Protein Expr Purif 2003 Oct;31(2):311-7. 6. [Expression of recombinant human stem cell factor in insect cells]. Wei Sheng Wu Xue Bao 2000 Feb;40(1):44-9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.