Technical Data
Stromal Cell-Derived Factor-1 alpha, Recombinant, Human (SDF1A, Stromal Cell-derived Factor 1, SDF1, SDF-1, hSDF-1, Chemokine (C-X-C motif) Ligand 12, C-X-C Motif Chemokine 12, CXCL12, Intercrine Reduced in Hepatomas, IRH, hIRH, Pre-B Cell Growth-stimulat
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to 68aa of human Stromal Cell-Derived Factor-1 alpha expressed in E.coli is a non-glycosylated, polypeptide chain.

Molecular Weight:

Biological Activity:
The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80nug/ml.

Dimers and Aggregates:

Protein Content:
Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 1.06 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SDF-1 as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Chemokine receptor CXCR4-dependent internalization and resecretion of functional chemokine SDF-1 by bone marrow endothelial and stromal cells. Nat Immunol 2005 Oct;6(10):1038-46. 2. Complement C3a enhances CXCL12 (SDF-1)-mediated chemotaxis of bone marrow hematopoietic cells independently of C3a receptor. J Immunol 2005 Sep 15;175(6):3698-706. 3. RGS16 is a negative regulator of SDF-1-CXCR4 signaling in megakaryocytes. Blood 2005 Nov 1;106(9):2962-8. 4. Causal pathways of the effects of age and the CCR5-Delta32, CCR2-64I, and SDF-1 3'A alleles on AIDS development. J Acquir Immune Defic Syndr 2005 Jul 1;39(3):321-6. 5. Stromal cell-derived factor-1 (SDF-1) alleles and susceptibility to breast carcinoma. Cancer Lett 2005 Jul 28;225(2):261-6. 6. Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis.
Stem Cells 2005 Aug;23(7):879-94.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.