Technical Data
Substance P (NK, Neurokinin)
Substance P is a tachykinin family neuropeptide that functions as a neurotransmitter and neuromodulator. In the CNS, Substance P is involved in the regulation of mood, anxiety, sleep, saiety, reinforcement, respiratory rhythm and pain processing. In nociception, the neuropeptide is released at local spinal cord synapse. Recent research now shows substance P is potentially involved in endothelial cell differentiation as well as diverse roles in neuroinflammatory response. The endogenous receptor for Substance P is neurokinin 1 receptor (NK1 receptor), which belongs to the tachykinin receptor sub-family of GPCRs.

Suitable for use in Immunohistochemistry and RIA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:100. Antigen retrieval with Citrate pH 6.0 is recommended
RIA: 1:10,000. 80% binding detects ~5pg/ml.
Optimal dilutions to be determined by the researcher.

Recommended Control:
Spinal Cord

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not Determined
Substance P (Accession# NP_003173)
Supplied as a liquid in 0.09% sodium azide.
Recognizes human Substance P. Species Crossreactivity: rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fusco, F.R., et al (2003). Neurosci. Let. 339: 53-56. 2. Brain Research (1986) 377:199-203. 3. Biology of Reproduction (1985) 33:471-476. 4. Life Sciences (1985) 37:16271632.