Technical Data
S8060-10M
Superoxide Dismutase 3 (Superoxide Dismutase 3 Extracellular, SOD 3, SOD3, Extracellular Superoxide Dismutase [Cu Zn], Extracellular Superoxide Dismutase Precursor, EC SOD, MGC20077)
Description:
SOD3 is a member of the superoxide dismutase protein family. SODs are antioxidant enzymes that catalyse the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. SOD3 is thought to protect the brain, lungs and other tissues from oxidative stress. It is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A small percentage of SOD3 is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the extracellular matrix.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:4000
Immunohistochemistry (Frozen/Paraffin): 1:100-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to aa18-37, WTGEDSAEPNSDSAEWIRD, of human extracellular Superoxide Dismutase, conjugated to diphtheria toxin.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
Specificity:
Recognizes human extracellular superoxide dismutase. Stains prominently specific human airway (probably Clara cells) and alveolar type II cells and some tubular cells in the kidney.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hjalmarsson K, et al. Proc. Natl. Acad. Sci. U.S.A. 84:6340-6344(1987). ; 2. Folz R.J, et al. Genomics 22:162-171(1994). ; 3. Liu T, et al. J. Proteome Res. 4:2070-2080(2005). ; 4. Sandstrom J, et al. J. Biol. Chem. 269:19163-19166(1994). ; 5. Yamada H, et al. Jpn. J. Hum. Genet. 40:177-184(1995). ; 6. Adachi T, et al. Biochem. J. 313:235-239(1996). ; 7. Adachi T, et al. J. Biochem. 120:184-188(1996). ; 8. Folz R, et al. Genomics 22(1): 162-171(1994).