Technical Data
S8060-10M
Superoxide Dismutase 3 (Superoxide Dismutase 3 Extracellular, SOD 3, SOD3, Extracellular Superoxide Dismutase [Cu Zn], Extracellular Superoxide Dismutase Precursor, EC SOD, MGC20077)
Description:
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homotetramer.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Cellular Localization:
Secreted protein; extracellular space. Note=99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in quilibrium between the plasma and the endothelium.

Tissue Specificity:
Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. POLYMORPHISM: The variant Gly-231 which is found in about 2.2% of individual displays a 10-fold increased plasma EC-SOD content due to reduced heparin-binding affinity and thus the impairment of its binding ability to endothelial cell surface. Belongs to the Cu-Zn superoxide dismutase family.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide (WTGEDSAEPNSDSAEWIRD) corresponding to the amino acids 18-37 of human extracellular superoxide dismutase (SOD3) conjugated to diphtheria toxin has been used as the immunogen.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity:
This antibody has been shown to be specific for extracellular superoxide dismutase (SOD3) protein. Species Crossreactivity: Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hjalmarsson K, et al. Proc. Natl. Acad. Sci. U.S.A. 84:6340-6344(1987). ; 2. Folz R.J, et al. Genomics 22:162-171(1994). ; 3. Liu T, et al. J. Proteome Res. 4:2070-2080(2005). ; 4. Sandstrom J, et al. J. Biol. Chem. 269:19163-19166(1994). ; 5. Yamada H, et al. Jpn. J. Hum. Genet. 40:177-184(1995). ; 6. Adachi T, et al. Biochem. J. 313:235-239(1996). ; 7. Adachi T, et al. J. Biochem. 120:184-188(1996). ; 8. Folz R, et al. Genomics 22(1): 162-171(1994).