Technical Data
S8060-25B
Superoxide Dismutase, Mn (SOD Mn)
Description:
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4).
There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16kD each are linked by two cysteines forming an intra-subunit disulfide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80kD. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30kD and it exists only in the extracellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml was sufficient for detection of Mn SOD in 20ug of rat brain tissue.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
25ul4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Rat Mn SOD
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.0, 0.1% sodium azide, 50% glycerol.
Specificity:
Recognizes rat Mn Superoxide Dismutase (SOD) at 25kD. Species Crossreactivity: Human, Mouse, Bovine, Canine, Chicken, Drosophila, Guinea Pig, Porcine, Hamster, Monkey, Rabbit, Sheep, Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Adachi, T., et al., Clin. Chim. Acta. 212: 89-102 (1992). 2. Barrister, J.V., et al., Crit. Rev. Biochem. 22: 111-180 (1987). 3. Furukawa, Y. & O Halloran, T., Antioxidants & Redox Signaling. 8: 847-867 (2006). 4. Gao, B., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 284: L917-L925 (2003). 5. Hassan, H.M., Free Radical Biol. Med. 5: 377-385 (1988). 6. Kurobe, N. & Kato, K., Biomed. Rsch. 12: 97-103 (1991). 7. Wispe, J.R., et al., BBA. 994: 30-36 (1989). 8. Xiao-Hong, L., et al., Brain Res. 625: 29-37 (1993).